Restriction Enzyme Analysis – Varying Enzymatic Conditions Date: February 2, 2017
Students will test and analyze four factors that can affect the function of restriction enzymes. Students will present their results to their classmates. Procedures used include: agarose gel electrophoresis and restriction enzymes (EcoRI, HinDIII, and BamHI) digestion of DNA.
Bacterial Transformation Date: March 2, 2017
Students genetically engineer bacteria through transformation with plasmid DNA. Students are directly involved in experimental design and the designation of controls. This laboratory can be adjusted to suit the academic level of the class.
Polymerase Chain Reaction Date: NOT AVAILABLE
Students analyze DNA in food using the polymerase chain reaction (PCR) and agarose-gel electrophoresis. Classroom discussion links these techniques with applications in forensics, genetic testing and genetic engineering
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